Rapid identification of Mycobacterium avium ssp paratuberculosis laboratory strains by IS900-Nested polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Detection of Mycobacterium avium subspecies paratuberculosis using nested polymerase chain reaction (nPCR)
Mycobacterium avium subspecies paratuberculosis, the cause of paratuberculosis (Johne’s disease) is a chronic debilitating infection in ruminants. The disease is one of the most widespread bacterial infections of ruminants throughout the world. Recently, the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the prese...
متن کاملIdentification of Mycobacterium avium subspecies paratuberculosis by Polymerase Chain Reaction in Blood and Semen of a Bull with Clinical Paratuberculosis
Mycobacterium avium subspecies paratuberculosis was identified by nested polymerase chain reaction in the blood and semen of a 4year-old Holstein breeding bull that exhibited clinical signs of paratuberculosis and was serologically positive for the organism by agar gel immunodiffusion and ELISA, although three fecal cultures were negative for the organism. The animal subsequently died, but a ne...
متن کاملMycobacterium avium ssp. paratuberculosis vs Crohn’s Disease
Crohn's disease (CD) is a chronic inflammatory bowel disease, with the potential to affect any segment of the gastrointestinal tract. Despite the great advances in recent decades, which provided a better understanding of the pathogenesis of the disease, it is yet to be completely elucidated. Presence of genetic factors, luminal factors such as microflora and factors related to the intestinal ba...
متن کاملRAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN REACTION
We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...
متن کاملExploring MALDI-TOF MS approach for a rapid identification of Mycobacterium avium ssp. paratuberculosis field isolates.
AIMS The aim of the study was to explore the suitability of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for a rapid and correct identification of Mycobacterium avium ssp. paratuberculosis (MAP) field isolates. METHODS AND RESULTS MALDI-TOF MS approach is becoming one of the most popular tests for the identification of intact bacterial cells whic...
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ژورنال
عنوان ژورنال: International Journal of Mycobacteriology
سال: 2016
ISSN: 2212-5531
DOI: 10.1016/j.ijmyco.2016.10.018